Yes. The intent is to stop any protest before it may happen. If the umpire has the knowledge of an ineligible pitcher or player, do not allow this. At the plate meeting before the game, the Umpire-in-Chief should determine if there are any ineligible pitchers on either team. If the opposing manager or any of the umpires has reason to believe that one team is not properly identifying player/pitcher eligibility, then the Umpire-in-Chief should ensure that the manager in question understands the eligibility requirements of Regulation VI.
If there is still a question of eligibility, consult with the game support staff (Scorekeeper and Official Pitch Count Recorder) and any Board member seeking resolution. Since, this is a serious safety issue, if unable to clearly determine the correct pitcher eligibility, suspend the game and get proper documentation. If documentation is available in a reasonable amount of time, proceed. If not, do not play until resolved. Regardless of the outcome, file a game report with the League President.